camera and filter wheel lambda 10-3 Search Results


excitation emission filter wheels lambda-10-3 controller  (Sutter Instrument)
90
Sutter Instrument excitation emission filter wheels lambda-10-3 controller
Excitation Emission Filter Wheels Lambda 10 3 Controller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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camera imagemx2  (Hamamatsu)
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Hamamatsu camera imagemx2
Camera Imagemx2, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ten position filter wheels  (Sutter Instrument)
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Sutter Instrument ten position filter wheels
Ten Position Filter Wheels, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c9100-02 camera  (Hamamatsu)
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Hamamatsu c9100-02 camera
C9100 02 Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hamamatsu orca-er camera  (Hamamatsu)
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Hamamatsu hamamatsu orca-er camera
Hamamatsu Orca Er Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ccd camera  (Nikon)
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Nikon ccd camera
Ccd Camera, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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digital ccd camera  (Hamamatsu)
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Hamamatsu digital ccd camera
Digital Ccd Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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orca-r2 ccd camera  (Hamamatsu)
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Hamamatsu orca-r2 ccd camera
Orca R2 Ccd Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retiga r3 ccd camera  (QImaging)
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QImaging retiga r3 ccd camera
Retiga R3 Ccd Camera, supplied by QImaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scmos camera flash 4.0  (Hamamatsu)
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Hamamatsu scmos camera flash 4.0
(a) Working principle of axially swept light-sheet microscopy: a thin light-sheet is scanned in its propagation direction. The rolling shutter readout of an <t>sCMOS</t> <t>camera,</t> adjusted to the size of the beam waist, is tightly synchronized to the light sheet scan. (b) Experimentally measured PSF of Airy scan confocal microscopy, conventional light-sheet fluorescence microscopy (LSFM), and ctASLM. LSFM and ctASLM PSFs are shown for NA 0.4 (indicated with “1”) and 0.7 objectives (indicated with “2”). (c-d) Maximum intensity projections of CLARITY cleared cortical Thy1-eYFP neurons as imaged by LSFM and ctASLM, respectively. (e) An XY view of a section 2.5mm from the top surface of a Thy1-eGFP PEGASOS cleared brain. (f) Zoom-in of the red box with a rendered neuron. Insets provide magnified views of synaptic spines. (g) Volume rendering of a mouse kidney labeled with Flk1-GFP. Inset shows a 3D rendering of endothelial cells in one selected glomerulus. (h) Maximum intensity projection of a mouse tibia bone marrow plug. (i) Zoom-in of magenta box shows alpha-catulin-GFP+ cells (green, Alexa Fluor 488), Lepr+ niche cells (red, tdTomato) and cKit+ cells (grey, Alexa Fluor 647). Alpha-catulin-GFP+/cKit+ hematopoietic stem cells (HSC) are labeled with cyan spheres. (j&k) are the volume-rendering of boxed region in (h), showing progenitor cells in grey and niche cells in red. All biological data acquired with NA 0.4 objectives. Scale bars are: (b) 3 μm, (c,d) 20 μm, (e) 1 mm, (g) 500 μm, (h) 1 mm, (i) 200 μm, (j) 40×40×40 μm 3 , (k) 5 μm.
Scmos Camera Flash 4.0, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axiovert 200 m wide-field microscope  (Carl Zeiss)
90
Carl Zeiss axiovert 200 m wide-field microscope
(a) Working principle of axially swept light-sheet microscopy: a thin light-sheet is scanned in its propagation direction. The rolling shutter readout of an <t>sCMOS</t> <t>camera,</t> adjusted to the size of the beam waist, is tightly synchronized to the light sheet scan. (b) Experimentally measured PSF of Airy scan confocal microscopy, conventional light-sheet fluorescence microscopy (LSFM), and ctASLM. LSFM and ctASLM PSFs are shown for NA 0.4 (indicated with “1”) and 0.7 objectives (indicated with “2”). (c-d) Maximum intensity projections of CLARITY cleared cortical Thy1-eYFP neurons as imaged by LSFM and ctASLM, respectively. (e) An XY view of a section 2.5mm from the top surface of a Thy1-eGFP PEGASOS cleared brain. (f) Zoom-in of the red box with a rendered neuron. Insets provide magnified views of synaptic spines. (g) Volume rendering of a mouse kidney labeled with Flk1-GFP. Inset shows a 3D rendering of endothelial cells in one selected glomerulus. (h) Maximum intensity projection of a mouse tibia bone marrow plug. (i) Zoom-in of magenta box shows alpha-catulin-GFP+ cells (green, Alexa Fluor 488), Lepr+ niche cells (red, tdTomato) and cKit+ cells (grey, Alexa Fluor 647). Alpha-catulin-GFP+/cKit+ hematopoietic stem cells (HSC) are labeled with cyan spheres. (j&k) are the volume-rendering of boxed region in (h), showing progenitor cells in grey and niche cells in red. All biological data acquired with NA 0.4 objectives. Scale bars are: (b) 3 μm, (c,d) 20 μm, (e) 1 mm, (g) 500 μm, (h) 1 mm, (i) 200 μm, (j) 40×40×40 μm 3 , (k) 5 μm.
Axiovert 200 M Wide Field Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lambda 10-3 controller  (Sutter Instrument)
90
Sutter Instrument lambda 10-3 controller
(a) Working principle of axially swept light-sheet microscopy: a thin light-sheet is scanned in its propagation direction. The rolling shutter readout of an <t>sCMOS</t> <t>camera,</t> adjusted to the size of the beam waist, is tightly synchronized to the light sheet scan. (b) Experimentally measured PSF of Airy scan confocal microscopy, conventional light-sheet fluorescence microscopy (LSFM), and ctASLM. LSFM and ctASLM PSFs are shown for NA 0.4 (indicated with “1”) and 0.7 objectives (indicated with “2”). (c-d) Maximum intensity projections of CLARITY cleared cortical Thy1-eYFP neurons as imaged by LSFM and ctASLM, respectively. (e) An XY view of a section 2.5mm from the top surface of a Thy1-eGFP PEGASOS cleared brain. (f) Zoom-in of the red box with a rendered neuron. Insets provide magnified views of synaptic spines. (g) Volume rendering of a mouse kidney labeled with Flk1-GFP. Inset shows a 3D rendering of endothelial cells in one selected glomerulus. (h) Maximum intensity projection of a mouse tibia bone marrow plug. (i) Zoom-in of magenta box shows alpha-catulin-GFP+ cells (green, Alexa Fluor 488), Lepr+ niche cells (red, tdTomato) and cKit+ cells (grey, Alexa Fluor 647). Alpha-catulin-GFP+/cKit+ hematopoietic stem cells (HSC) are labeled with cyan spheres. (j&k) are the volume-rendering of boxed region in (h), showing progenitor cells in grey and niche cells in red. All biological data acquired with NA 0.4 objectives. Scale bars are: (b) 3 μm, (c,d) 20 μm, (e) 1 mm, (g) 500 μm, (h) 1 mm, (i) 200 μm, (j) 40×40×40 μm 3 , (k) 5 μm.
Lambda 10 3 Controller, supplied by Sutter Instrument, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda 10-3 controller/product/Sutter Instrument
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Image Search Results


(a) Working principle of axially swept light-sheet microscopy: a thin light-sheet is scanned in its propagation direction. The rolling shutter readout of an sCMOS camera, adjusted to the size of the beam waist, is tightly synchronized to the light sheet scan. (b) Experimentally measured PSF of Airy scan confocal microscopy, conventional light-sheet fluorescence microscopy (LSFM), and ctASLM. LSFM and ctASLM PSFs are shown for NA 0.4 (indicated with “1”) and 0.7 objectives (indicated with “2”). (c-d) Maximum intensity projections of CLARITY cleared cortical Thy1-eYFP neurons as imaged by LSFM and ctASLM, respectively. (e) An XY view of a section 2.5mm from the top surface of a Thy1-eGFP PEGASOS cleared brain. (f) Zoom-in of the red box with a rendered neuron. Insets provide magnified views of synaptic spines. (g) Volume rendering of a mouse kidney labeled with Flk1-GFP. Inset shows a 3D rendering of endothelial cells in one selected glomerulus. (h) Maximum intensity projection of a mouse tibia bone marrow plug. (i) Zoom-in of magenta box shows alpha-catulin-GFP+ cells (green, Alexa Fluor 488), Lepr+ niche cells (red, tdTomato) and cKit+ cells (grey, Alexa Fluor 647). Alpha-catulin-GFP+/cKit+ hematopoietic stem cells (HSC) are labeled with cyan spheres. (j&k) are the volume-rendering of boxed region in (h), showing progenitor cells in grey and niche cells in red. All biological data acquired with NA 0.4 objectives. Scale bars are: (b) 3 μm, (c,d) 20 μm, (e) 1 mm, (g) 500 μm, (h) 1 mm, (i) 200 μm, (j) 40×40×40 μm 3 , (k) 5 μm.

Journal: Nature methods

Article Title: Light-Sheet Microscopy of Cleared Tissues with Isotropic, Subcellular Resolution

doi: 10.1038/s41592-019-0615-4

Figure Lengend Snippet: (a) Working principle of axially swept light-sheet microscopy: a thin light-sheet is scanned in its propagation direction. The rolling shutter readout of an sCMOS camera, adjusted to the size of the beam waist, is tightly synchronized to the light sheet scan. (b) Experimentally measured PSF of Airy scan confocal microscopy, conventional light-sheet fluorescence microscopy (LSFM), and ctASLM. LSFM and ctASLM PSFs are shown for NA 0.4 (indicated with “1”) and 0.7 objectives (indicated with “2”). (c-d) Maximum intensity projections of CLARITY cleared cortical Thy1-eYFP neurons as imaged by LSFM and ctASLM, respectively. (e) An XY view of a section 2.5mm from the top surface of a Thy1-eGFP PEGASOS cleared brain. (f) Zoom-in of the red box with a rendered neuron. Insets provide magnified views of synaptic spines. (g) Volume rendering of a mouse kidney labeled with Flk1-GFP. Inset shows a 3D rendering of endothelial cells in one selected glomerulus. (h) Maximum intensity projection of a mouse tibia bone marrow plug. (i) Zoom-in of magenta box shows alpha-catulin-GFP+ cells (green, Alexa Fluor 488), Lepr+ niche cells (red, tdTomato) and cKit+ cells (grey, Alexa Fluor 647). Alpha-catulin-GFP+/cKit+ hematopoietic stem cells (HSC) are labeled with cyan spheres. (j&k) are the volume-rendering of boxed region in (h), showing progenitor cells in grey and niche cells in red. All biological data acquired with NA 0.4 objectives. Scale bars are: (b) 3 μm, (c,d) 20 μm, (e) 1 mm, (g) 500 μm, (h) 1 mm, (i) 200 μm, (j) 40×40×40 μm 3 , (k) 5 μm.

Article Snippet: The detection arm consisted of an identical microscope objective (Cleared Tissue Objective, Advanced Scientific Imaging), a tube lens (ITL200-A, ThorLabs), a 4-position filter wheel (LB10-WHS4 and a Lambda 10–3, Sutter Instruments), and a sCMOS camera (Flash 4.0, Hamamatsu Corporation).

Techniques: Microscopy, Confocal Microscopy, Fluorescence, Labeling